Journal: International Journal of Medical Sciences

Article Title: Comparison the Prognostic Value of Galectin-3 and Serum Markers of Cardiac Extracellular Matrix Turnover in Patients with Chronic Systolic Heart Failure

doi: 10.7150/ijms.8083

Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for prediction mortality. The area under the curve (AUC) of log MMP-2, log TIMP-1, log BNP, log PIIINP, log Gal-3, log PINP, and log MMP-9 were 0.786, 0.699, 0.636, 0.625, 0.607, 0.519, and 0.367, respectively. Abbreviations: BNP= brain natriuretic peptide; Gal-3= Galectin-3; HF=heart failure; LVEF= left ventricular ejection fraction; MMP = matrix metalloproteinase; PINP = type I amioterminal propeptide of procollagen; PIIINP = type III amioterminal propeptide of procollagen; TIMP = tissue inhibitor of metalloproteinase.

Article Snippet: TIMP-1 was measured by an ELISA kit (DTM100, R & D systems, Minneapolis, USA).

Techniques:

Journal: International Journal of Medical Sciences

Article Title: Comparison the Prognostic Value of Galectin-3 and Serum Markers of Cardiac Extracellular Matrix Turnover in Patients with Chronic Systolic Heart Failure

doi: 10.7150/ijms.8083

Figure Lengend Snippet: Kaplan-Meier analysis of cumulative rates of survival in HF patients with higher or lower levels of serum Gal-3 or cardiac ECM markers. The p value of Gal-3, PINP, PIIINP, TIMP-1, MMP-2, MMP-9, and BNP were 0.153, 0.708, 0.154, 0.028, 0.001, 0.501, and 0.483, respectively. Abbreviations: BNP= brain natriuretic peptide; ECM= Extracellular matrix; Gal-3= Galectin-3; HF=heart failure; LVEF= left ventricular ejection fraction; MMP = matrix metalloproteinase; PINP = type I amioterminal propeptide of procollagen; PIIINP = type III amioterminal propeptide of procollagen; TIMP = tissue inhibitor of metalloproteinase.

Article Snippet: TIMP-1 was measured by an ELISA kit (DTM100, R & D systems, Minneapolis, USA).

Techniques:

Journal: International Journal of Medical Sciences

Article Title: Comparison the Prognostic Value of Galectin-3 and Serum Markers of Cardiac Extracellular Matrix Turnover in Patients with Chronic Systolic Heart Failure

doi: 10.7150/ijms.8083

Figure Lengend Snippet: Kaplan-Meier analysis of cumulative rates of HF admission-free survival in HF patients with higher or lower levels of serum Gal-3 or cardiac ECM markers. The p value of Gal-3, PINP, PIIINP, TIMP-1, MMP-2, MMP-9, and BNP were 0.166, 0.624, 0.639, 0.085, <0.001, 0.624, and 0.684, respectively. Abbreviations: BNP= brain natriuretic peptide; ECM= Extracellular matrix; Gal-3= Galectin-3; HF=heart failure; MMP = matrix metalloproteinase; PINP = type I amioterminal propeptide of procollagen; PIIINP = type III amioterminal propeptide of procollagen; TIMP = tissue inhibitor of metalloproteinase.

Article Snippet: TIMP-1 was measured by an ELISA kit (DTM100, R & D systems, Minneapolis, USA).

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts

doi: 10.3389/fbioe.2020.00428

Figure Lengend Snippet: RT-PCR primers.

Article Snippet: Supernatant of cell lysates was collected for each of six cell types [HFF (NC/SI/OE) and HSF (NC/SI/OE)], and the levels of MMP2, TGFβ1, and TIMP1 were quantified using ELISA kits as per manufacturers’ instructions (Human Matrix Metalloproteinase 2/Gelatinase A (MMP-2) ELISA Kit, Cat.#CSB-E04675h, CUSABio, Wuhan, Hubei, China; Human TGF-beta1 ELISA Kit, Cat.#EK1811, MultiSciences, Hangzhou, Zhejiang, China; Human TIMP1 ELISA Kit, Cat.#EK11382, MultiSciences).

Techniques: Control

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Proteomics Identifies Circulating TIMP-1 as a Prognostic Biomarker for Diffuse Large B-Cell Lymphoma

doi: 10.1016/j.mcpro.2023.100625

Figure Lengend Snippet: The reagents and tools used in this study

Article Snippet: TIMP-1 concentration was determined with an ELISA kit from Sino Biological according to the manufacturer’s instructions.

Techniques: Software, Microarray, Mass Spectrometry, Enzyme-linked Immunosorbent Assay

Journal: Lupus science & medicine

Article Title: Proteomics uncovers ICAM2 (CD102) as a novel serum biomarker of proliferative lupus nephritis.

doi: 10.1136/lupus-2024-001446

Figure Lengend Snippet: Figure 2 ELISA validation of three serum biomarkers using an independent cohort and their correlation with clinical indices. (A–C) A cohort of 80 serum samples: 30 healthy controls (HC, green) and 50 patients with lupus nephritis (LN, red) were tested by ELISA for the levels of ICAM2, TIMP1 and THBS1. (D) The discriminatory abilities of the three serum markers were assessed in distinguishing patients with LN from HCs using receiver operating characteristic (ROC) curve analysis. (E–K) The correlation of ELISA-assayed serum ICAM2 levels with clinical indices in LN. r denotes Spearman’s correlation coefficient. ****p<0.0001. AUC, area under the curve.

Article Snippet: Serum samples for TIMP1 (CUSABIO, Catalogue No CSB- E08003h, Wuhan, China) and THBS1 (CUSABIO, Catalogue No CSB- E08763h, Wuhan, China) were diluted 1:100 and 1:400, respectively, whereas the ICAM2 (BOSTER, Catalogue No EK0999, Peachtree Corners, Georgia) ELISA assays used serum at 1:5 dilution.

Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery